ABSTRACT
To explore the effects of microRNA-150 deletion on the development and homeostasis of regulatory T cells (Treg),γδT cells,NK and NKT cells.Methods:microRNA-150 knockout mice were used and microRNA-150 expression was detected by Real-time PCR.The numbers of Treg ,γδT,NK and NKT cells in the thymus and spleen of normal control and microRNA-150 knockout mice were detected by Flow cytometry.Cell apoptosis was detected by Annexin V staining , and cell proliferation was detected by 5-Bromo-2-deoxyUridine ( Brdu ) incorporation.Results: microRNA-150 deletion did not affect the development and homeostasis of regulatory T cells (Treg) andγδT cells.However,microRNA-150 deletion resulted in a significant reduction of the NK and thymic NKT cell number.In addition, microRNA-150 deleted NK and NKT cells showed an arrested developmental and maturational phenotype with a reduced expression of NK 1.1 and CD122.Moreover , cell apoptosis was significantly increased in microRNA-150 deleted NK and thymic NKT cells ,while a lower cell proliferation rate was shown in the microRNA-150 deleted NK but not NKT cells.Conclusion: CD122 may play an important role in the development and homeostasis of mouse NK and NKT cells regulated by microRNA-150.
ABSTRACT
ObjectiveTo invest the situation and to improve the education on biosafety among medical graduate students.MethodsTaking the medical graduate students of Sun Yat-sen University enrolled in fall 2008 as investigators,we implemented investigation on biosafety by a self-designed biosafety awareness questionnaires.The investigation included four fields,the legislation and institution on biosafety,the laboratory operating rules and regulations,the consciousness of protection and the emergency response skill on biosafety.ResultsWe got the present situations of the medical graduate students on biosafety which showed that it was urgent for students to strengthen the biosafety training and education.ConclusionWe tried to provide reference for establishing the educational system on laboratory biosafety based the Sun Yat-sen University's practice.